cd1® igs mouse  (Charles River Laboratories)

Journal: EMBO Molecular Medicine

Article Title: Uncovering biomarkers for chronic toxoplasmosis detection highlights alternative pathways shaping parasite dormancy

doi: 10.1038/s44321-025-00252-0

Figure Lengend Snippet: ( A ) Western blot membranes from IAA-treated and untreated extracts were probed with antibodies from mice chronically infected with cystogenic T. gondii strains. Results were consistent across replicates, and a representative blot is shown. ( B ) Silver staining of fraction 17 (F17) from S200 gel filtration chromatography (full column fractions shown in Fig. ). ( C ) Western blot of F17 probed with sera from naive mice and mice chronically infected with T. gondii . ( D ) BSM was immunopurified using anti-Flag antibodies, and Western blots of the purified protein were probed with sera from chronically infected mice bearing T. gondii cysts (CD1 infected with ME49, NMRI, and BALB/c infected with 76 K) or from NMRI mice infected with the cyst-free strain CTG. ( E ) Alphafold model of the BSM protein (generated via the EBI/Alphafold server), displayed as a cartoon and color-coded from the N-terminal (blue) to the C-terminal (red). ( F ) Alphafold position error heatmap illustrating the predicted subdomains of BSM. .

Article Snippet: CD1 ( M. musculus ) , Charles River , CD1® IGS Mouse.

Techniques: Western Blot, Infection, Silver Staining, Filtration, Chromatography, Purification, Generated

Journal: EMBO Molecular Medicine

Article Title: Uncovering biomarkers for chronic toxoplasmosis detection highlights alternative pathways shaping parasite dormancy

doi: 10.1038/s44321-025-00252-0

Figure Lengend Snippet: ( A ) NMRI mice ( n = 15) were injected intraperitoneally with 5.10 4 tachyzoites of the 76K-GFP-luc WT or Δ bsm strains, and their survival was monitored. Statistical analysis using log-rank Mantel–Cox and Gehan–Breslow–Wilcoxon tests showed no significant difference ( P = 0.3496). ( B ) Microscopy analysis of cyst morphology in homogenized brain suspensions from mice infected with 76K-GFP-luc and Δ bsm strains. Over 20 cysts were examined per strain, and representative images are presented. ( C , D ) Parasitic loads in parasite per brain (qPCR count) and expression levels of miR-155 and miR-146a were assessed in mice infected by 76 K WT ( n = 7) versus 76 K Δ bsm ( n = 7). Statistical significance was calculated using a nonparametric Mann–Whitney test. ( E ) Parasitic loads (parasites per brain, quantified by qPCR) were measured in mice ( n = 74) of various backgrounds (NMRI, Balb/C, CD1) infected with cystogenic strains (76 K, ME49; in red), low cystogenic strains (CTG, Pruku80; in green), 76 K Δbsm (in blue), and 76 K or Pruku80 Δbcla (in purple). ( F ) BSM and BCLA ELISA serology in mice ( n = 83) is shown using the same color coding as in panel ( E ). Statistical significance was calculated using a nonparametric Mann–Whitney test. ( G ) ROC (Receiver Operating Characteristic) curves for BSM ELISA serology (blue) and BCLA ELISA serology (purple). ROC curves are graphical representations that evaluate the diagnostic performance of a test by plotting the true positive rate (sensitivity) against the false positive rate (1 − specificity) at various thresholds. ( C – F ) The error bars indicate the standard error of the mean (SEM). .

Article Snippet: CD1 ( M. musculus ) , Charles River , CD1® IGS Mouse.

Techniques: Injection, Microscopy, Infection, Expressing, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay, Diagnostic Assay

Journal: Cell Reports Medicine

Article Title: Caspase-8 inhibition improves the outcome of bacterial infections in mice by promoting neutrophil activation

doi: 10.1016/j.xcrm.2023.101098

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Article Snippet: Mouse: CD1 IGS , Charles River Laboratories , Strain Code 022.

Techniques: Purification, Blocking Assay, Control, Functional Assay, Virus, Recombinant, Sterility, Saline, Protease Inhibitor, Western Blot, Enzyme-linked Immunosorbent Assay, Red Blood Cell Lysis, Gene Expression, LDH Cytotoxicity Assay, Bicinchoninic Acid Protein Assay, Staining, Reverse Transcription, Software, Real-time Polymerase Chain Reaction, Membrane, Spectrophotometry, Flow Cytometry

Journal: Molecular Systems Biology

Article Title: A single‐cell RNA labeling strategy for measuring stress response upon tissue dissociation

doi: 10.15252/msb.202211147

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Article Snippet: CD1 feeder cells , charles river , CD‐1® IGS Mouse.

Techniques: Cell Culture, RNA Extraction